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finalize manual_transform tutorial
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martinschorb committed Jul 8, 2024
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8 changes: 7 additions & 1 deletion tutorials/volume_registration.md
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Expand Up @@ -15,6 +15,7 @@ This tutorial will show how to generate a project from multimodal volume images
- you should get a Hyperstack image with 4 channels. (MitoTracker, Agglutinin, GFP-TGN46, and Hoechst)
- adjust the lookup table for each channel to fit the fluorophore. (`Image > Lookup Tables`)
- Currently, the MoBIE project creator only supports single channels, so we have to split the fluorescence stack into separate channels using `Image > Color > Split Channels`.

---

- create a new MoBIE project: type "mobie" in the search bar or choose `Plugins > MoBIE > Create > Create New MoBIE project...`
Expand Down Expand Up @@ -50,6 +51,7 @@ This tutorial will show how to generate a project from multimodal volume images

![multiview.png](tutorial_images/multiview.png)

### Manual registration of sources

- right click the image "registration manual", select the EM image as we want to keep the multi-channels where they are.
![img.png](tutorial_images/manual_transform.png)
Expand All @@ -74,4 +76,8 @@ This tutorial will show how to generate a project from multimodal volume images
Note that when clicking `Accept manual transform` the single transformed source will be saved as a new view. In case you like to transform more.

- To save a complete view with all sources (channels + EM), do `right click > save current view` and save your view as desired.
![img.png](tutorial_images/saveView.png)
![img.png](tutorial_images/saveView.png)

### (optional) registration using BigWarp

- `right click > Registration - BigWarp`

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