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Error when running debugSingleSV #298

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wongwsw opened this issue Oct 14, 2022 · 0 comments
Open

Error when running debugSingleSV #298

wongwsw opened this issue Oct 14, 2022 · 0 comments

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@wongwsw
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wongwsw commented Oct 14, 2022

Hi,

I was following the steps in https://github.com/Illumina/manta/blob/master/docs/developerGuide/debugSingleSV.md to debug a single SV that was missed by Manta, but could be called by CNVpytor.

All steps worked until the last step (step 2), where I encountered the following error:
FATAL_ERROR: No output file specified to SynchronizedOutputStream
cmdline: /lustre/tools/manta-1.6.0.release_src/libexec/GenerateSVCandidates --align-stats PGPC37/01alignment/v2/workspace/alignmentStats.xml --graph-file PGPC37/01alignment/v2/workspace/svLocusGraph.bin --ref ref/hg19/hg19.fa --candidate-output-file PGPC37/01alignment/v2/tmp.vcf --chrom-depth PGPC37/01alignment/v2/workspace/chromDepth.txt --align-file PGPC37/01alignment/PGPC37.dedup.merged.bam --min-pass-qual-score 10 --edge-prob 0.05
version: 1.6.0
buildTime: 2022-05-23T01:24:52.715164Z
compiler: g++-4.8.5

Please advise on how to troubleshoot.

Many thanks!

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